嘌呤霉素二盐酸盐；；Puromycin ；Puromycin dihydrochloride分子式：C22H29N7O5.2(HCl)分子量：544.43
CAS 号：58-58-2
质量标准：≥98%
TESTS
SPECIFICATIONS
Appearance
White to slightly beige powder
UV/Vis
UV max 218,276nm
Purity(HPLC)
≥98%
物理性状及指标：外观：……………………类白色粉末熔点：……………………178-180 °C℃溶解性：…………………溶于水(50 mg/ml),乙醇(~1 mg/ml), DMSO (~13 mg/ml), DMF (~14 mg/ml)IC50：……………………半数致死剂量(LD50)经口-老鼠- 720 mg/kg使用方法：（来自公开文献，仅供参考）储存液配置：Puromycin 溶解于无菌细胞培养级1M HEPES buffer (MA0036)或者PBS1X(MA0015)，配置成浓度为10mg/ml. 无菌微膜过滤。然后根据自身实验要求进行稀释或者使用筛选条件：Escherichia coli（大肠杆菌）：Puromycin is poorly active on E. coli. However, puromycin-resistant transformants can be selected in LB agar medium supplemented with 100-125 μg/ml of puromycin.Mammalian cells (哺乳动物细胞) The working concentrations of puromycin for mammalian cell lines range from 1 to 10 μg/ml. In a starting experiment we recommend to determine optimal concentrations of antibiotic required to kill your host cell line. Puromycin quickly kills eukaryotic cells that do not contain the pac gene. Dying cells detach from the plates, allowing easy and early identification of transformant clones. Suggested working conditions for selection in some mammalian cells are listed below.
Cell lineMediumPuromycin conc.B16 (Mouse melanocytes)RPMI1-2 μg/mlHEK293 (Human kidney cells)DMEM0.5-10 μg/mlHeLa (Human uterine cells)DMEM1-10 μg/mlMEF (Mouse fibroblasts)DMEM1-5 μg/ml用途及描述：科研试剂，广泛应用于分子生物学，药理学等科研方面。嘌呤霉素(puromycin;PM )是一种蛋白质合成抑制剂,它具有与tRNA分子末端类似的结构,能够同氨基酸结合，代替氨酰化的tRNA同核糖体的A位点结合，并掺入到生长的肽链中。虽然嘌呤霉素能够同A位点结合,但是不能参与随后的任何反应,因而导致蛋白质合成的终止并释放出C-末端含有嘌呤霉素的不成熟的多肽。由于嘌呤霉素对原核和真核生物的翻译过程均有干扰作用，故难于用做抗菌药物，主要用作研究蛋白质合成的生物化学工具。有人试用于肿瘤治疗。储存条件：-20° C，避光防潮密闭干燥。
运输条件：2~8℃运输
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